basdan.blogg.se

Flowjo 10 compensation
Flowjo 10 compensation










MultiGraph Overlay FlowJo can display multiple subpopulations of a single sample overlayed on one for visual comparison. The following examples will all be demoed with overlaid populations, but remember that the Multigraph function can be utilized with plots that have not been overlaid. (As stated above, the plot can be of a single population/sample or of an overlay of multiple samples/popualtions. To access the Multigraph interface in version 10, place a plot in the layout editor and right click on the plot. Simply drag a sample into the layout editor and then drag another sample or population from the workspace on top of an existing graph in the layout editor. Batch the layou Overlays are done in the layout editor. Using the Legend, right-click the control data layer, and click Set As Control.

flowjo 10 compensation

Create the initial overlay by dragging one population on top of another in the Layout Editor. Drop the second graph (or more, if you had multiple nodes selected in the workspace) on top of the original, and an overlay is automatically created Here is the process: 1.

flowjo 10 compensation

As the mouse moves into the original graph item, you'll see that it highlights its borders to signify that it will accept the contents of the drag. Then drag a second on top of the first one. To create an overlay, drag a graph into the Layout Editor. On a parameter by parameter basis in univariate histograms, by binning two histograms together to reveal a bivariate dot plot, or even applying machine learning to generate derived parameters representing embedded space in a single plot. Overlays give researchers a powerful way to visualize comparisons between populations.

flowjo 10 compensation

This greatly aids in the comparison of results produced by the various modeling platforms, and makes for a killer graph to show at your next. Starting in FlowJo v10.6, platform figures (Kinetics, Cell Cycle, and Proliferation) can be overlaid in the Layout Editor by dragging and dropping the platform nodes on top of one another.












Flowjo 10 compensation